Laboratory investigation of patients with high lactate dehydrogenase activity, by lactate dehydrogenase isozyme analysis.
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چکیده
منابع مشابه
A Human Trophoblastic Isozyme (Lactate Dehydrogenase-Z) Associated with Choriocarcinoma1
A unique electrophoretic form of lactate dehydrogenase (LDH-Z), formerly observed in a choriocarcinoma cell line (JEG 3) and first-trimester placenta, has been shown to be the same as that produced in hydatidiform mole and term placenta. We have also observed LDH-Z in second-trimester placenta, cho riocarcinoma metastasized to the liver, and five of five addi tional independently derived (from ...
متن کاملA human trophoblastic isozyme (lactate dehydrogenase-Z) associated with choriocarcinoma.
A unique electrophoretic form of lactate dehydrogenase (LDH-Z), formerly observed in a choriocarcinoma cell line (JEG-3) and first-trimester placenta, has been shown to be the same as that produced in hydatidiform mole and term placenta. We have also observed LDH-Z in second-trimester placenta, choriocarcinoma metastasized to the liver, and five of five additional independently derived (from di...
متن کاملKinetic properties of rabbit testicular lactate dehydrogenase isozyme.
The additional lactate dehydrogenase isozyme from rabbit testis and sperm, lactate dehydrogenase isozyme-1 (B4) from heart, and lactate dehydrogenase isozyme-5 (A4) from liver have been separated and partially purified by preparative starch gel electrophoresis. Studies performed on these fractions comprised K, and optimum substrate concentration for pyruvate, a-ketobutyrate, and lactate, inhibi...
متن کاملMeasurement of total lactate dehydrogenase activity.
Lactate dehydrogenase (LD: EC 1.1.1.27) is the most important clinically of several dehydrogenases occurring in human serum. Lactate dehydrogenase is cytoplasmic in its cellular location and in any one tissue is composed of one or two of five possible isoenzymes. While many of its clinical applications involve quantification of one or more specific serum isoenzymes, an estimate of total LD is r...
متن کاملOptimum reaction conditions for human lactate dehydrogenase isoenzymes as they affect total lactate dehydrogenase activity.
Optimum reaction conditions at 30#{176} ± 0.5#{176} for two continuous spectrophotometric assay procedures, lactate to pyruvate (LP) and pyruvate to lactate (PL), were determined with respect to pH at 30#{176} (pH30) substrate concentration, and coenzyme concentration for the human LDH isoenxymes. For the P L procedure, broad pH30 optima were within the range of 7.20-7.40 for all the LDH isoenz...
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ژورنال
عنوان ژورنال: SEIBUTSU BUTSURI KAGAKU
سال: 2007
ISSN: 0031-9082,1349-9785
DOI: 10.2198/sbk.51.243